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Quantitative Biology > Quantitative Methods

Title: Accurate evaluation of size and refractive index for spherical objects in quantitative phase imaging

Abstract: Measuring the average refractive index (RI) of spherical objects, such as suspended cells, in quantitative phase imaging (QPI) requires a decoupling of RI and size from the QPI data. This has been commonly achieved by determining the object's radius with geometrical approaches, neglecting light-scattering. Here, we present a novel QPI fitting algorithm that reliably uncouples the RI using Mie theory and a semi-analytical, corrected Rytov approach. We assess the range of validity of this algorithm in silico and experimentally investigate various objects (oil and protein droplets, microgel beads, cells) and noise conditions. In addition, we provide important practical cues for future studies in cell biology.
Comments: 14 pages, 10 figures, 1 table
Subjects: Quantitative Methods (q-bio.QM); Biological Physics (physics.bio-ph)
Cite as: arXiv:1803.06410 [q-bio.QM]
  (or arXiv:1803.06410v1 [q-bio.QM] for this version)

Submission history

From: Paul Müller [view email]
[v1] Fri, 16 Mar 2018 21:51:33 GMT (970kb,D)